¨  The Structural Biochemistry Ph.D. Program

ˇ  Prior studies and degrees needed

For a successful participation in this program, M.Sc. (in biology and chemistry), M.D., D.V. or D.D. degrees are accepted on the condition that the curriculum of the undergraduate and graduate programs of applicant included chemistry, biochemistry and molecular biology as major subjects.

ˇ  Features of the program

§  Objectives  This program offers training in that field of biology, which aims the understanding of physiological processes at molecular-structural level.  Therefore it mainly involves molecular-structural and enzymological analysis, exploration of structure-function relationships and studying intermolecular interactions.

§  Curriculum  The curriculum is assembled so that students can gain a detailed practical experience in state-of-art methodology, that is used in the research project they join. At the same time, they can get a firm knowledge, through seminar series and regular as well as one-time courses, about of all those methods that are relevant for a complex structural-biochemical approach of biological problems. These include protein purification, protein chemistry and sequence analysis, structure investigation with, UV-, fluorescence- and NMR- spectroscopy, calorimetry, X-ray crystallography and molecular modeling, as well as steady state and fast kinetic analysis of enzymes. In most of the projects, students use molecular biology techniques for cloning, site directed mutagenesis and heterologous expression of proteins, as well as for the analysis of transcriptom and proteom.

§  Currently available projects  Students in the Structural Biochemistry Program are recommended to participate in the work of research groups at Eötvös Loránd University, but it is also possible that they join groups in research institutions of the Hungarian Academy of Sciences which participate in the Program. The research groups at the University pursue projects in studying proteolytic enzymes and motor proteins and processes related to these proteins.

The molecular-biological problems in the focus of our projects:

The mechanism of action of serine proteases,

The structural basis of inhibition of proteolytic enzymes,

The physiological function of specific proteases in the nervous tissue,

The involvement of proteolytic systems in the infection process of pathogen bacteria,

Motor proteins involved in the intracellular transport,

Mechanism of myosin motor proteins,

Regulation of myosin motors,

The control muscle contraction,

Interdependence of mechanical and biochemical kinetic properties of molecular motors,

Diversity of myosin-based mechanisms of motility,

The structural background and thermodynamics of amyloid formation,

Directed evolution approaches in biotechnology and protein science.



ˇ          Program head:

László Gráf, D.Sc. professor

ˇ          Program coordinator to be contacted:

István Venekei Ph.D. associate professor